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KMID : 0545120050150020412
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Journal of Microbiology and Biotechnology
2005 Volume.15 No. 2 p.412 ~ p.420
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Process Development for Effective Denitrification by Biofilter Using Loess Ball
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Choi DB
Lee DB/Cha WS
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Abstract
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In order to investigate factors affecting the denitrification in the F-STEP PROCESS using a loess ball as support media and Pseudomonas DWC 17-8 calcining temperature loess ball size pH nitrate concentration working temperature and inhibitor were studied in batch mode using synthetic sludge. A 5-10 mm of loess ball (960oC of calcining temperature) was the most suitable for denitrification. When the initial pH was increased from 3.0 to 7.0 the removal efficiency of nitrate was increased. Specifically at initial pH of 7.0 the maximum removal efficiency of nitrate was 5.0 mg/min. When the initial concentration of nitrate was increased from 100 to 400 mg/l the removal efficiency of nitrate was proportional to the concentration of nitrate. The maximum removal efficiency of nitrate was 5.72 mg/min at 400mg/l of initial concentration. When the operating temperature was increased from 10 to 30oC the removal efficiency of nitrate was increased from 0.76 to 6.15 mg/min and at above 40oC of operating temperature it was decreased from 4.0 to 2.0 mg/min. Among the various inhibitors higher than 10-1 M of sodium azide abolished this reaction completely. When the KCN concentration was above 10-1 M the reaction was inhibited completely. In the case of 24-dinitrophenol and sodium sulphide it was inhibited at above 10-2 M completely. For testing the various flow orders of the F-STEP PROCESS for effective denitrification using practical wastewater continuous experiments under the optimum conditions were carried out for 60 days. Among the various processes the PROCESS A gave the highest efficiencies of denitrification nitrification and total nitrogen (TN) removal with 86.5 89.5 and 90% respectively. For scale-up in the PROCESS A real farm wastewater was used and pilot tests carried out for 90 days. The denitrification efficiency was 97.5% which was increased by 12.7%. The efficiencies of TN removal and nitrification were 96.6 and 70.0% respectively. The removal efficiency of chemical oxygen demand (COD) was 63.7% which was increased by 20%.
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